Overview of the reporter genes and reporter mouse models
WEBApr 19, 2018 · In these multi‐color fluorescent reporter mice, 4 fluorescent reporter genes (GFP, YFP, RFP and CFP) can be expressed in a random manner after Cre‐mediated DNA excisions and inversions. Szyska et al 69 generated dual‐luciferase reporter mouse model expressing an NFAT‐dependent click‐beetle luciferase and a renilla luciferase.
Fluorescent transgenic mouse models for whole-brain imaging in …
WEBSep 23, 2022 · In this review, we discuss the recent advances in the development of fluorescent transgenic mouse models that overcome to some extent the technical and conceptual limitations and tradeoffs between different strategies. We also discuss the potential use of these strains for understanding disease.
A global double-fluorescent Cre reporter mouse - PubMed
WEBHere we describe mT/mG, a double-fluorescent Cre reporter mouse that expresses membrane-targeted tandem dimer Tomato (mT) prior to Cre-mediated excision and membrane-targeted green fluorescent protein (mG) after excision.
Establishment of transgenic fluorescent mice for labeling …
WEBMar 7, 2024 · In our study, we generated transgenic mice by expressing fluorescent proteins to label synapses and skip the immunofluorescence staining step during synaptogenic adhesion molecules screening. We chose SYT1 as a media to transport tdTomato to the synapse by fusing tdTomato to the C-tail of SYT1.
Reporter Mouse Lines for Fluorescence Imaging - Abe - 2013 ...
WEBSeveral groups have reported their success in generating different reporter mouse lines that express a variety of fluorescent markers for imaging. However, there is currently no established database of the reporter mouse lines available for live imaging, such as the Cre transgenic lines (Cre-X-Mice).
Imaging In Mice With Fluorescent Proteins: From Macro To …
WEBFeb 22, 2008 · With the dual-color cancer cells and a highly sensitive small animal imaging system, subcellular dynamics can now be observed in live mice in real time. Fluorescent proteins thus enable both macro and micro imaging technology and thereby provide the basis for the new field of in vivo cell biology.
Imaging the mouse as a whole - Nature Methods
WEBFeb 27, 2019 · Clearing methods render opaque tissues and even whole animal bodies transparent. This transparency allows the imaging of fluorescently labeled cells or structures within samples. Yet...
Transgenic mice encoding modern imaging probes: Properties …
WEBMay 24, 2022 · We describe the application of transgenic mice expressing fluorescent proteins for tracking protein expression patterns, apoptotic events, tissue differentiation and regeneration, neurogenesis, tumorigenesis, and cell fate mapping.
In vivo fluorescence imaging: success in preclinical imaging paves …
WEBOct 15, 2022 · In this review, we discuss the latest advances in in vivo fluorescence imaging technologies, NIR fluorescent probes, and current and future clinical applications. Advances in diagnostic imaging have provided unprecedented opportunities to detect diseases at early stages and with high reliability.
Fast confocal fluorescence imaging in freely behaving mice
WEBNov 2, 2018 · Here, we present a novel fiberscope system that provides fast (up to 200 Hz) background-free fluorescence imaging in freely behaving mice over a field of view of diameter 230 μm.