Cell therapy is an emerging novel treatment option for an array of diseases, ranging from genetic disorders to cancer. As the cell therapy field expands, there is a growing demand for safe and ...

When a cell is able to take up free DNA or RNA, it is termed “competent”. Some cell types or microbial species, such as Bacillus subtilis, 11 are naturally competent and they can be induced to uptake ...

To increase the levels of pulmonary gene transfer by nonviral vectors, we have adopted electroporation protocols for use in the lung. A volume of 100–200 μl of purified plasmid DNA suspended in saline ...

Electroporation — the use of high-voltage electric pulses to introduce DNA/RNA/proteins into cells — has made cellular engineering and gene expression easier than ever imagined. One version of this ...

Thermo Fisher Scientific’s new Gibco CTS Xenon Electroporation System aims to provide easier scale up for cell therapies, from clinical development to commercial manufacturing. Thermo Fisher ...

Using an electric current to deliver the CRISPR/Cas9 system, in order to engineer genetic changes in laboratory mice, is highly efficient and significantly improves the system's throughput over the ...

Graduate students Mingde ‘Jack’ Zheng and Joseph Sherba have developed a novel, microfluidic platform for monitoring electroporation and molecular delivery at the single cell-level as part of a NSF ...

It is well understood that external electric fields can affect the integrity of cell membranes. This is due to an inherent difference between the electric potentials of the interior of a cell membrane ...

Scientists are refining electroporation methods to improve CRISPR/Cas9 gene editing efficiency while preserving cell health. By adjusting buffer composition, pulse parameters, and delivery formats, ...

3-dimensional rendering of a cell passing through the electroporation zone while a pulse is being applied. The electric field lines which normally do not penetrate an insulating cell are able to ...