If you can't delete an ISO file as the file is open in your System, check if it is mounted as a drive or not. Uses these proven fixes to resolve the issue.

Why Cant I Delete, Edit, or Rename a File That Is in Use? Windows prevents you from deleting, moving, or renaming files that are in use to keep your computer system stable.

We explain why you might not be able to delete OneDrive files or folders on your Windows PC and the best solutions to fix the issue.

Then I typed "fsutil reparsepoint delete <file name>" (without the quotes) then hit enter. I was then able to go back to the file and delete it normally. Not open for further replies.