Genome editing technologies like CRISPR-Cas9 have transformed biology, medicine, and agriculture, but concerns remain about unintended edits at off-target DNA sites. These off-target effects can cause ...

Detection of the off-target effects of base editors is important for identifying their safety risks but current methods for understanding their global activities have limitations in terms of ...

As a fast and easy-to-use gene-editing technique, CRISPR is one of the biggest discoveries in 21 st century medical science, and an important facilitator of gene therapy development. Several gene ...

Researchers introduce Variant-aware Cas-OFFinder, a web-based tool that improves CRISPR off-target predictions by incorporating individual genetic differences. By reconstructing allele-specific ...

10F.9G2, a monoclonal antibody with high antibody-dependent cellular cytotoxicity, has an off-target effect that involves the reduction of CD8+ T cells. This surpasses the on-target effect, namely ...