Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...
The receptors and proteins were filtered from multiple datasets (Oncomine database) comparing prostate tumor tissue with normal tissue (based on Gene Ontology and statistical false-discovery rate ...